Warning: mysql_query() [function.mysql-query]: Unable to save result set in /www/users/HK591466/WEB/includes/db.inc.php on line 59
Database error: Invalid SQL: select * from pwn_comment where pid='454706' and iffb='1' order by id limit 0,10
MySQL Error: 996 (Query execution was interrupted, max_statement_time exceeded)
#0 dbbase_sql->halt(Invalid SQL: select * from pwn_comment where pid='454706' and iffb='1' order by id limit 0,10) called at [/www/users/HK591466/WEB/includes/db.inc.php:65] #1 dbbase_sql->query(select * from {P}_comment where pid='454706' and iffb='1' order by id limit 0,10) called at [/www/users/HK591466/WEB/comment/module/CommentContent.php:167] #2 CommentContent() called at [/www/users/HK591466/WEB/includes/common.inc.php:551] #3 printpage() called at [/www/users/HK591466/WEB/comment/html/index.php:13]
Warning: mysql_fetch_array(): supplied argument is not a valid MySQL result resource in /www/users/HK591466/WEB/includes/db.inc.php on line 72
网友点评-H, www.sigmaaldrich.com) was {used|utilized|employed|utilised|applied|made-缅甸万丰国际.13187589555
您好,欢迎光临!   [请登录]   [免费注册]
发布于:2019-5-15 20:51:43  访问:51 次 回复:0 篇
版主管理 | 推荐 | 删除 | 删除并扣分
H, www.sigmaaldrich.com) was {used|utilized|employed|utilised|applied|made
Protein determination The protein concentration was estimated by the Bradford (1976) strategy using bovine serum albumin (BSA) as a normal. Outcomes trans-3-Phenylacrylic acidmedchemexpress Mortality studies 1st, the toxicity of P. aeruginosa elastase B as much as the 7th instar larvae of G. mellonella was determined (Figure 1). 3 doses of purified elastase B (0.05, 0.1, and 0.two g/larvae) have been applied. Insects had been injected by means of the last pro eg and mortality prices had been determined more than a 48 hour period. The mortality prices observed for larvae inoculated with elastase atJournal of Insect Science | www.insectscience.orgJournal of Insect Science: Vol. 12 | Short article 88 the concentration of 0.1 and 0.two g per larvae following 48 hours incubation had been 8 and 14 , respectively. Results indicated that PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/22937147 the doses of purified elastase B have been sublethal for G. mellonella larvae and these doses have been chosen for additional experiments. Impact of elastase B on hemolymph proteins/peptides profile Subsequent experiments investigated the susceptibility of proteins and peptides present in immune hemolymph of G.H, www.sigmaaldrich.com) was used as a standard for evaluation
H, www.sigmaaldrich.com) was utilized as a standard for evaluation of antibacterial activity Lysozyme activity Lysozyme activity in hemocyte ree hemolymph and in hemocyte ree hemolymph extract was detected employing agarose plates containing freeze ried Micrococcus luteus (Sigma) (Jarosz 1995). Every single effectively on the Petri dish was filled with 4 L sample, and after 24 hours incubation at 28 , the diameters on the M. luteus lytic zones were measured. The activity of lysozyme was calculated from a standard curve produced with EWL (Sigma; EC and expressed in g/mL EWL. Electrophoresis techniques and immunoblotting Polyacrylamide gel electrophoresis of protein samples was performed by tricine SDS-PAGE (16.five T, three C) based on Sch ger and Jagow (1987). Polypeptide bands in gels had been stained with Coomassie Brilliant Blue G-250 (Imperial Chemical Industries). IEF was performed with one hundred g of hemolymph extract protein utilizing the Protean IEF focusing program (Bio-Rad, www.biorad.com) based on the manufacturer‘s suggestions. The sample was suspended in rehydratation buffer (8.eight mol/L urea, 2 ,Andrejko and Mizerska-Dudka W/V CHAPS; 70 mmol/L DTT; 0.2 , W/V, Bio-Lytes) and loaded on 70 mm IPG strips (Bio-Rad). After separation of proteins within the first dimension, strips had been equilibrated twice for 15 min in equilibration buffer (6 mol/L urea; 20 , V/V, glycerol; 2 , W/V, SDS; 375 mmol/L Tris-HCL, pH eight.eight). The initial step was done in equilibration buffer with 130 mmol/L DTT; the second equilibration step contained 135 mmol/L iodoacetamide. PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/22291896 Then tricine SDS-PAGE and immunoblotting had been performed below the circumstances described above. The samples immediately after tricine and 2D electrophoresis were electroblotted onto Immobilon membranes (Millipore, www.millipore.com) for 90 min at 350 mA. For identification of apolipophorin-III, anti?G. mellonella apoLp-III antibodies (Agrisera, www.agrisera.com) were utilised. Alkaline phosphatase onjugated goat anti abbit IgGs have been utilized as secondary antibodies.
共0篇回复 每页10篇 页次:1/1
共0篇回复 每页10篇 页次:1/1
验 证 码